|
KMID : 0380019940090020115
|
|
Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.115 ~ p.121
|
|
|
Separation of Lentil Lectin Using Free-Flow Electrophoresis
|
|
|
|
|
Abstract
|
|
|
|
A purification device with 30-channel free-flow electrophoresis was assembled to treat samples of 240m1 volume for purification of lentil lectin(LcH) from lentil seeds with no impurities in a silverstained PAGIEF gel. HEPES(50mM)-Tris(5OmM), Cyclosen´ne(50mM)-urea (3M), Histidine(50mM)urea( 3M) were used as ampholytes, among which Histidine(50mM)-urea(3M) (pI 7.65) was found best in resolution. LcH is known to be present in the form of LcH-A, LcH-B and the complex of the two. The complex, howeve;disappeared when urea was added in the ampholytes, which suggested that the complete purification of two isolectins is possible using the present multistep purificaton device.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|